Commit ee116dfb authored by Sabina Köfler's avatar Sabina Köfler
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Add troubleshooting section

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......@@ -15,20 +15,20 @@ The scanner can be used in two modes:
The scanner can be used with slides and coverslips that meet the following specifications:
|| Slides | Coverglasses |
|--------------|-----------|------------|
| length |75.0 to 76.0 mm | max. 50 mm |
| Width |25.0 to 26.0 mm | max. 24 mm (recommended: 22 mm) |
|thickness |0.90 to 1.2 mm|No. 1 and No. 1.5 (0.13 to 0.16 mm and 0.16 to 0.19 mm)|
|Corners|45° bevelled corners|
|Edges|Sanded or cut|
| | Slides | Coverslips |
|-----------|----------------------|---------------------------------------------------------|
| length | 75.0 to 76.0 mm | max. 50 mm |
| Width | 25.0 to 26.0 mm | max. 24 mm (recommended: 22 mm) |
| thickness | 0.90 to 1.2 mm | No. 1 and No. 1.5 (0.13 to 0.16 mm and 0.16 to 0.19 mm) |
| Corners | 45° bevelled corners |
| Edges | Sanded or cut |
Slides covered with foil can also be used.
Slides covered with varnish must NOT be used. This is possible in principle, but the unit would have to be readjusted. If necessary, please contact Sabina Köfler.
### General
With HE staining, the contrast is very good and hardly any manual adjustment is required.
With H&E staining, the contrast is very good and hardly any manual adjustment is required.
With IHC slides the contrast is worse. These slides therefore require more manual intervention.
There are two programmes that are used:
......@@ -140,13 +140,13 @@ Simply create a document in advance with the following data:
An example document consisting of a rack with 5 slides could look like this:
|rack number (1-6)|slide position (1-25)|desired file name|barcode (optional)|profile name|illumination type (BrightField)|
|:----|:----|:----|:----|:----|:----|
|1|1|MyFileName1|123ABC|HE_20x_excellent|BrightField|
|1|2|MyFileName2|245DEF|HE_40x_excellent|BrightField|
|1|3|MyFileName3|678GHI|HE_40x_zstack_excellent|BrightField|
|1|4|MyFileName4|987JKL|IHC_20x_excellent|BrightField|
|1|5|MyFileName5|654MNO|IHC_40x_excellent|BrightField|
| rack number (1-6) | slide position (1-25) | desired file name | barcode (optional) | profile name | illumination type (BrightField) |
|:------------------|:----------------------|:------------------|:-------------------|:------------------------|:--------------------------------|
| 1 | 1 | MyFileName1 | 123ABC | HE_20x_excellent | BrightField |
| 1 | 2 | MyFileName2 | 245DEF | HE_40x_excellent | BrightField |
| 1 | 3 | MyFileName3 | 678GHI | HE_40x_zstack_excellent | BrightField |
| 1 | 4 | MyFileName4 | 987JKL | IHC_20x_excellent | BrightField |
| 1 | 5 | MyFileName5 | 654MNO | IHC_40x_excellent | BrightField |
> **Note**: A slide can also be included in the list more than once, for example if it is to be scanned with different profiles.
......@@ -208,7 +208,23 @@ The unit is maintained by Sanova. In case of a problem please contact Sabina Kö
## Troubleshooting
### Image artefacts
In case artefacts like those shown in the images below appear you need to check the profile's **Image settings > Use saved compensation image**.
Compensation images are reference images used for white balancing and shading.
If **Use saved compensation image** is checked, create a new one.
If it is unchecked: make sure that your slide has enough clean background available for the scanner to create a compensation image.
<img src="../img/artefacts01.png" width="300"/>
<img src="../img/artefacts02.png" width="300"/>
### Misc
To open the housing cover, loosen the four screws on the bottom of the housing.
If a scanner error occurs, check the plug connections.
## Appendix
......
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